Authors
Lucien Murhula NAMEGABE1*,2, Djibril FALL3, Yerim Mbagnick DIOP2,, Serigne Omar SARR2
1: Département de Pharmacie, Faculté des Sciences Pharmaceutiques et Santé Publique, Université Officielle de Bukavu, Bukavu, P.O. Box 570 Bukavu, République Démocratique du Congo
2: Laboratoire de Chimie Analytique et Bromatologie, Université Cheikh Anta Diop, BP 5005, Dakar, Sénégal
3: Laboratoire de Chimie Organique et Thérapeutique, Université Cheikh Anta Diop, BP 5005, Dakar, Sénégal
Correspondance: murhulan@yahoo.fr
Abstract
Nevirapine is an antiretroviral drug used in the prevention of mother-to-child transmission. High-performance liquid chromatography is the most widely recommended technique for quantitative analysis of nevirapine in pharmaceutical dosage forms and biological fluids. A new spectrofluorimetric method has been developed and validated for its determination. The aim of this study was to compare the performance of this new method with the reference method.
The following statistical tests and tools were used: the Fisher test to compare variances in nevirapine assay concentrations; the Strengths, Weaknesses, Opportunities and Threats approach; and the AGREE automated greening tool.
No significant differences were observed between the assay results obtained by the two analytical methods. A higher strength and opportunity score was observed for the spectrofluorimetric method than for the chromatographic method. The costs of reagents used and equipment purchased using chromatography were three to ten times higher than those using spectrofluorimetry. The total AGREE green score for spectrofluorimetry was 0.67, while that for HPLC was 0.48.
The spectrofluorimetric method was more economical, more ergonomic, more environmentally friendly and safer for human health than HPLC.
Key words: Spectrofluorometry, HPLC, Nevirapine, t-fisher, SWOT and AGREE-green
DOI | Volume | Number | Published | Open acess |
10.61585/pud-ijppna-v1i1a3 | 01 | 01 | 12-18-2023 | Yes |
Graphical abstract
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