Optimization and validation of a gas chromatography method for the determination of ethanol in blood samples
- ijppna
- 1 mai
- 2 min de lecture
Cheick Armand Noukami PALM1*, Moumouni BANDE1,2, Mahamadou MAIGA2, Naä-Imwine Stanislas Dimitri MEDA1, Odile GAMBO1, Arouna ZOUNGRANA1, Abdou Azize Wendketa OUEDRAOGO1, Soumaila KONATE1, Abdoul Karim SAKIRA2, Elie KABRE1,2
1: Agence Nationale pour la Sécurité Sanitaire de l’Environnement, de l’Alimentation, du Travail et des Produits de Santé (ANSSEAT); 09 BP: 24 Ouagadougou 09
2: Université Joseph KI-ZERBO, Unité de Formation et de Recherche en Sciences de Santé (UFR-SDS), Département des Sciences pharmaceutiques; 03 BP: 7021 Ouagadougou 03
*Correspondence:pancs2004@gmail.com
DOI:
Abstract: Ethanol is a psychoactive substance. Its abuse has health, regulatory and medico-legal consequences. Consequently, the determination of blood alcohol concentration (BAC) necessitates a highly sensitive and robust analytical procedure. To address this necessity, the National Agency for Health, Environmental, Food, Labor, and Health Product Safety (ANSSEAT) has developed a validated method for BAC quantification.
Blood samples were collected in tubes containing fluoride from 14 volunteers who did not drink alcohol and were then spiked with ethanol. Sample preparation involved treatment with trichloroacetic acid, incorporating n-propanol as an internal standard. Validation parameters were established in accordance with the protocol described by Feinberg et al. (1996).
Analytical measurements were conducted using gas chromatography equipped with a flame ionization detector (GC-FID), headspace injector and nitrogen gas. Data acquisition and processing were executed using ChemStation software and Microsoft® Excel 2016.
The response function adhered to a linear regression model (y = 1498.2x - 0.5957, r² = 0.9999). Results demonstrated linearity, precision, accuracy, selectivity, and robustness across the concentration range of 0.1 to 1 g/L. The validated limit of quantification was determined to be 0.03 g/L ± 0.0008, with a matrix effect correction factor of 0.89%.
The BAC determination method validated by ANSSEAT enhances the institution's analytical capabilities, thereby reinforcing its contribution to public health initiatives.
Keywords: Validation, Blood Alcohol Concentration (BAC), GC-FID
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